Problem Files Indexed Color and 10 12 14 Bit Depths Microscope Images

How to scale images from 10-, 12- and 14-bit to 16-bit in Photoshop. Images are typically at lower than 16-bit depths when acquiring scientific images, which means, on a practical level, these appear dark on the screen. Here’s how to scale images in Photoshop.

How to Open Indexed Color in GIMP

  1. Image > Mode: choose RGB Color (if in color) or Grayscale (if gray toned).

How to Open Indexed Color in Image J

Generally no need to change the mode.

How to Open 10-bit, 12-bit, 14-bit and 16-bit in GIMP (versions more recent than 2.9.2)

  1. Select Colors > Levels.
  2. Type “16” into the white level input slider box.
  3. Click OK

How to Open 10-, 12-, 14- and 16-bit images in Image J

Images will open, but will only appear perceptually correct if Appearance is set appropriately:
1. Edit > Options > Appearance
2. Uncheck all boxes (unless having a reason not to).
Choose 16-bit range dropdown and select appropriate bit depth*
* check bit depth of image by selecting Image > Show Info

See the Instruction Page for an index of all instructional videos and documents
See Image Ethics for image integrity information on how to avoid scientific misconduct

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