How to scale images from 10-, 12- and 14-bit to 16-bit in Photoshop. Images are typically at lower than 16-bit depths when acquiring scientific images, which means, on a practical level, these appear dark on the screen. Here’s how to scale images in Photoshop.
How to Open Indexed Color in GIMP
- Image > Mode: choose RGB Color (if in color) or Grayscale (if gray toned).
How to Open Indexed Color in Image J
Generally no need to change the mode.
How to Open 10-bit, 12-bit, 14-bit and 16-bit in GIMP (versions more recent than 2.9.2)
- Select Colors > Levels.
- Type “16” into the white level input slider box.
- Click OK
How to Open 10-, 12-, 14- and 16-bit images in Image J
Images will open, but will only appear perceptually correct if Appearance is set appropriately:
1. Edit > Options > Appearance
2. Uncheck all boxes (unless having a reason not to).
Choose 16-bit range dropdown and select appropriate bit depth*
* check bit depth of image by selecting Image > Show Info