How to open an image series (image sequence) in Photoshop for those who use confocal or widefield microscopes and take z-sections or time course. If images are from a confocal, it’s best to do steps in Image J (see Merging Fluorescent Images in Image J).
How to Open Image Series (image sequence) in GIMP
An image sequence in GIMP is generally done to subsequently create a GIF animation
1. File > Open as Layers
In Open Image box select all images from the image sequence
2. Optional: Image > Scale Image (to make smaller dimensions)
Specify image size in box in pixels
3. File > Export (to create GIF animation)
Put a “.gif” at end of file name
In next dialog box, if you want a continuous play, check Loop
Set delay at 12.**
*Image sizes are often too large for web output if images are too large (greater than 800 or so pixels in any dimension).
** Re-export if a delay of 12 is too slow or too fast and add delays iteratively.
Open Image Sequence in Image J
- File > Import > Image Sequence
- Select only the first file (001) in the image sequence
- Edit > Options > Appearance (set appearance to bit depth of images)
Open Confocal Series in Image J
File > Open*
In dialogue box click Virtual Stack (other settings can remain unchecked)
Edit > Options > Appearance (set appearance to bit depth of images)
* It’s assumed the opened file is in the manufacturer’s format (e.g. “.oib”)