Opening in Photoshop for Montaging Photostitching Microscope images

How to photostitch images in Photoshop, known as montaging among those who use microscopes in the sciences and as a Panorama. This is a means to put together several fields from a microscope at high magnification from multiple images into a single image.

How to Make a Panorama in GIMP

Download a plugin called Pandora and install. Search web for directions by using search terms “Panorama” and “GIMP.”

How to Make a Montage (Panorama, Photostitched Image) in Image J

  1. Begin with no images open
  2. File > Import > Image Sequence
  3. Plug-Ins > Stitching > deprecated > Stitch Directory with Images (unknown configuration).
    Select the directory; images must be same size and numbered left to right, then down (001_, 002_, etc.).
    Give the image an output file name, and start
  4. Edit > Options > Appearance: choose bit depth to match images.

*Several other plugins are available in ImageJ for stitching. Iteratively choose one that works for you. You may have difficulties with fluorescent and darkfield images.

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