How to reduce or remove hot pixels, shot noise and other instances when overly bright pixels neighbor pixels at uniform tones, typical of images with low fluorescent labeling.
How to Correct for Hot Pixels in GIMP
The steps are manual and take a great deal of time. Best to use Image J.
How to Correct for Hot Pixels in Image J
Under Process > Noise > Remove Outliers
In box, check Preview
Set Radius (typically 1 or 2)
Set Threshold (typically 100 – 128 for fluorescence)
Set Which Outliers (Bright or Dark).